王连艳-中国科学院大学-UCAS
[中文]
[English]
研究领域
招生信息
教育背景
工作经历
专利与奖励
出版信息
科研活动
基本信息
王连艳 女 博导 中国科学院过程工程研究所电子邮件： wanglianyan@ipe.ac.cn通信地址： 北京市海淀区中关村北二街1号中国科学院过程工程研究所邮政编码： 100190
研究领域
（1）新型纳微颗粒疫苗佐剂构建及其免疫增强机制研究。针对乙肝表面抗原构建了具有质子海绵效应的纳微颗粒，利用抗原的溶酶体逃逸提升细胞免疫功能，并基于此研发治疗性乙肝疫苗，进行免疫增强机制探索和治疗效果评价；针对HPV抗原，设计了具有pH敏感等相应释放的新型纳微颗粒，实现细胞和体液免疫的双重提升，与公司合作开发基于纳微颗粒的新型宫颈癌疫苗制剂；针对流感裂解抗原设计和构建了一系列新型纳微颗粒，开展相关免疫学评价，并研究免疫增强机制。同时，还系统研究了纳微颗粒理化性质对免疫学行为的影响，深化纳微颗粒佐剂设计。
（2）蛋白质药物微包埋中活性保持机制研究及其口服蛋白递送系统构建。针对微包埋过程中乳化过程、固化过程，系统研究了蛋白在微包埋过程中的失活原因，并提出了一系列的解决策略，实现了微包埋过程中蛋白多肽等活性药物的高活性保持，并针对口服蛋白多肽给药，设计构建了能够穿透粘液层的靶向杯状细胞的纳微颗粒口服给药体系，大大提高了药物的口服生物利用度；针对口服疫苗免疫效果不理想的问题，构建了具有M细胞靶向的脂质球递送系统，口服免疫获得了良好的效果。
（3）抗癌药物那为载体设计与制备研究。针对难溶抗肿瘤药物喜树碱等，设计和构建增加难溶药物溶出度及其具有靶向传输作用的纳微载体，研究抑瘤效果；同时，采用软模板技术，将哪容药物纳米化，大大提高了难溶药物的水溶性，而且可通过表面接枝进一步实现药物的靶向递送。
招生信息
招生专业
081703-生物化工086000-生物与医药
招生方向
纳微球作为疫苗佐剂及肿瘤免疫制剂研究蛋白质药物微包埋研究抗癌药物纳微载体设计与制备研究
教育背景
2002-09--2006-06 中国科学院过程工程研究所 博士学位1997-09--2000-06 东北林业大学 硕士学位1993-09--1997-06 东北林业大学 学士学位
工作经历
工作简历
2016-08~现在, 中国科学院过程工程研究所, 研究员2009-08~2016-07,中国科学院过程工程研究所, 副研究员2006-07~2009-07,中国科学院过程工程研究所, 助理研究员2000-07~2002-08,轻工业环境保护研究所, 中级工程师
社会兼职
2019-04-30-今,中国生物医学工程学会纳米医学分会, 高级会员2017-02-01-今,中国颗粒学会团体标准委员会, 委员2014-09-05-今,中国颗粒学会生物专业委员会, 委员2012-09-05-今,中国颗粒学会, 青年理事
专利与奖励
奖励信息
（1） 尺寸均一、可控的乳液微球和微囊, 二等奖, 国家级, 2009（2） 尺寸均一、可控的微球和微囊的制, 一等奖, 省级, 2006
专利成果
[1] 丁钢强, 霍军生, 王连艳, 黄建, 张贵锋, 唐艳斌, 曹科, 崔迎彬, 曹政. 一种载鱼油或藻油微胶囊及其制备方法和用途. CN: CN112106991A, 2020-12-22.[2] 张贵锋, 王连艳, 张博, 杨婷媛, 苏志国, 马光辉. 一种海藻酸钠-琼脂糖微球及其制备方法和应用. CN: CN106890159B, 2020-11-10.[3] 王连艳, 刘美娟, 杨婷媛. 阳离子脂质纳米疫苗佐剂及其制备方法. CN: CN111467487A, 2020-07-31.[4] 丁钢强, 霍军生, 王连艳, 黄建, 张贵锋, 唐艳斌, 曹科, 崔迎彬, 曹政. 一种载益生菌的微胶囊的制备方法及由其制得产品和应用. CN: CN110946287A, 2020-04-03.[5] 霍军生, 王连艳, 黄建, 杨婷媛, 张贵锋, 刘辉, 张博. 一种纳米级硫酸亚铁营养补充剂微囊化产品及其制备工艺. 中国: CN105477014B, 2018.04.13.[6] 马光辉, 王连艳, 苏志国, 刘媛, 杨婷媛, 周炜清. 一种W/O型疫苗油佐剂制剂及其制备方法. 中国: CN105148266B, 2018-09-04.[7] 王连艳, 刘永江, 贾成成, 马光辉, 陈健平, 潘勇昭, 银飞, 王雅君. 一种具有治疗或预防HPV病毒的疫苗组合物及其应用. 中国: CN104338126B, 2018-05-04.[8] 王慧梅, 王连艳, 韩淑兰, 张贵锋, 杨婷媛. 一种集化疗与免疫疗法为一体的纳米制剂及制备方法. 中国: CN106902095A, 2017-06-30.[9] 王连艳, 马光辉, 刘婍, 杨婷媛, 周炜清. 一种胞内pH响应聚乳酸类纳微球及其制备方法. 中国: CN106031794A, 2016-10-19.[10] 霍军生, 王连艳, 黄建, 杨婷媛, 张贵锋, 刘辉, 张博. 一种新型微纳米级鱼油/藻油微胶囊及其制备工艺. 中国: CN105533691A, 2016-05-04.[11] 马光辉, 王连艳, 苏志国, 杨婷媛, 马童童, 周炜清. 一种载活性药物的聚合物脂质球及其制备方法. 中国: CN105287431A, 2016-02-03.[12] 马光辉, 吕丕平, 魏炜, 王连艳, 周炜清, 苏志国. 一种装载难溶性药物的壳聚糖-壳聚糖衍生物纳米球、制备方法及其作为口服制剂的应用. 中国: CN102641245A, 2012.08.22.[13] 王连艳, 马光辉, 宋春艳. 一种胃部靶向药物载体及其制备方法. 中国: CN102652833A, 2012-09-05.[14] 马光辉, 吕丕平, 王连艳, 魏炜, 周炜清, 苏志国. 一种装载难溶性抗肿瘤药物的壳聚糖-羧化壳聚糖纳米球、制备方法及其应用. 中国: CN102389572A, 2012-03-28.[15] 王连艳, 马光辉, 杨娟娟, 张岳玲, 杨婷媛, 周炜清. 一种10-羟基喜树碱的纳微给药体系及其制备方法. 中国: CN102349871A, 2012-02-15.[16] 马光辉, 苏志国, 魏炜, 王连艳, 卫强. 荧光壳聚糖微球的制备方法及其在示踪剂领域的应用. 中国: CN101148511B, 2010-10-20.[17] 马光辉, 王连艳, 苏志国, 吕丕平, 魏 炜, 卫 强, 张岳玲. 壳聚糖纳微球产品及其制备方法. 中国: CN101675996, 2010-03-24.[18] 马光辉, 苏志国, 王连艳. 一种尺寸均一、包埋率高、药物活性保持率高的壳聚糖载药微球及其制备方法. 中国: CN100356980, 2007-12-26.[19] 马光辉, 苏志国, 王连艳. 一种尺寸均一的壳聚糖微球和微囊及其制备方法. 中国: CN1319637, 2007-06-06.[20] 马光辉, 苏志国, 王连艳, 周青竹. 一种尺寸均一的琼脂糖凝胶微球及其制备方法. 中国: CN1304101, 2007-03-14.
出版信息
发表论文
[1] Yongjie Chi, Peng Sun, Yuan Gao, Jing Zhang, Lianyan Wang. Ion Interference Therapy of Tumors Based on Inorganic Nanoparticles. Biosensors[J]. 2022, 12(2): 100-, https://www.mdpi.com/2079-6374/12/2/100/htm.[2] Han, Shulan, Ma, Wenyan, Jiang, Dawei, Sutherlin, Logan, Zhang, Jing, Lu, Yu, Huo, Nan, Chen, Zhao, Engle, Jonathan W, Wang, Yanping, Xu, Xiaojie, Kang, Lei, Cai, Weibo, Wang, Lianyan. Intracellular signaling pathway in dendritic cells and antigen transport pathway in vivo mediated by an OVA@DDAB/PLGA nano-vaccine. JOURNAL OF NANOBIOTECHNOLOGY[J]. 2021, 19(1): 394-425, https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-021-01116-8.[3] Han, Shulan, Wang, Wenjie, Wang, Shengfang, Yang, Tingyuan, Zhang, Guifeng, Wang, Di, Ju, Ruijun, Lu, Yu, Wang, Huimei, Wang, Lianyan. Tumor microenvironment remodeling and tumor therapy based on M2-like tumor associated macrophage-targeting nano-complexes. THERANOSTICS[J]. 2021, 11(6): 2892-2916, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806477/.[4] 王连艳. Micro- and nano-encapsulated hybrid system (MNEHDS): a novel approach for colon-targeted oral delivery of berberine. Molecular Pharmaceutics. 2021, [5] Zhang, Lingzhi, Li, Mingyan, Zhang, Guiqiu, Gao, Changxing, Wang, Shengfang, Zhang, Tingting, Ma, Chen, Wang, Lianyan, Zhu, Qing. Micro- and Nanoencapsulated Hybrid Delivery System (MNEHDS): A Novel Approach for Colon-Targeted Oral Delivery of Berberine. MOLECULAR PHARMACEUTICS[J]. 2021, 18(4): 1573-1581, https://www.webofscience.com/wos/woscc/full-record/WOS:000637870700007.[6] 王连艳. 艾塞那肽口服给药PLGA/SA 微球的制备. 维吾尔医药. 2020, [7] 刘美娟, 王连艳, 杨婷媛, 朱艳华. DDAB/PLA纳米疫苗佐剂放大工艺研究及初步安全性评估. 中国医药工业杂志[J]. 2019, 50(6): 653-659, http://lib.cqvip.com/Qikan/Article/Detail?id=7002271859.[8] Han, Shulan, Wang, Wenjie, Wang, Shengfang, Wang, Shuo, Ju, Ruijun, Pan, Zihao, Yang, Tingyuan, Zhang, Guifeng, Wang, Huimei, Wang, Lianyan. Multifunctional biomimetic nanoparticles loading baicalin for polarizing tumor-associated macrophages. NANOSCALE[J]. 2019, 11(42): 20206-20220, [9] Wang, Huimei, Han, Shulan, Wang, Lianyan, Yang, Tingyuan, Zhang, Guifeng, Yu, Lian, Zhao, Yue. Dual-function baicalin and baicalin-loaded poly(lactic-co-glycolic acid) nanoparticles: Immune activation of dendritic cells and arrest of the melanoma cell cycle at the G2/M phase. PARTICUOLOGY[J]. 2018, 37: 64-71, http://lib.cqvip.com/Qikan/Article/Detail?id=675485769.[10] Yang, Meiyang, Yang, Tingyuan, Jia, Jilei, Lu, Ting, Wang, Hailin, Yan, Xueying, Wang, Lianyan, Yu, Lian, Zhao, Yue. Fabrication and characterization of DDAB/PLA-alginate composite microcapsules as single-shot vaccine. RSC ADVANCES[J]. 2018, 8(24): 13612-13624, http://dx.doi.org/10.1039/c8ra00013a.[11] 马文颜, 康磊, 王连艳, 霍焱, 申镐源, 王艳萍. （131）~I标记卵清蛋白及其载（131）~I-OVA的PLGA/DDAB纳米粒SPECT显像. 科学技术与工程[J]. 2018, 18(8): 27-33, http://www.irgrid.ac.cn/handle/1471x/2497186.[12] Liu, Qi, Chen, Xiaoming, Jia, Jilei, Lu, Ting, Yang, Tingyuan, Wang, Lianyan. Potential Hepatitis B Vaccine Formulation Prepared by Uniform-Sized Lipid Hybrid PLA Microparticles with Adsorbed Hepatitis B Surface Antigen. MOLECULAR PHARMACEUTICS[J]. 2018, 15(11): 5227-5235, http://ir.ipe.ac.cn/handle/122111/26782.[13] Jia, Chengcheng, Yang, Tingyuan, Liu, Yongjiang, Zhu, Ali, Yin, Fei, Wang, Yajun, Xu, Lan, Wang, Yan, Yan, Mei, Cai, Qingman, Liang, Xiaoxu, Ju, Ruijun, Chen, Jianping, Wang, Lianyan. A Novel Human Papillomavirus 16 L1 Pentamer-Loaded Hybrid Particles Vaccine System: Influence of Size on Immune Responses. ACS APPLIED MATERIALS & INTERFACES[J]. 2018, 10(42): 35745-35759, http://ir.ipe.ac.cn/handle/122111/26470.[14] 张宇, 王连艳, 王艳萍. 嗜酸乳杆菌微胶囊制备及工艺优化. 食品与发酵工业[J]. 2018, 44(6): 132-138, http://lib.cqvip.com/Qikan/Article/Detail?id=675974871.[15] 王红芳, 王连艳, 杨婷媛, 殷瑛, 阎雪莹. DDAB/PLA纳米颗粒佐剂浓度对免疫效果的影响. 现代预防医学[J]. 2018, 45(14): 2603-2609, http://lib.cqvip.com/Qikan/Article/Detail?id=675735424.[16] 邱越佳, 孟丹, 杨婷媛, 王连艳, 朱艳华. 口服载胰岛素PLGA纳米球的制备及初步药效学评价. 中国医药工业杂志[J]. 2018, 49(5): 614-621, http://ir.ipe.ac.cn/handle/122111/28898.[17] Guo, Fangxia, Liu, Yongdong, Zhang, Chun, Wang, Qi, Wang, Lianyan, Gao, Yuhui, Bi, Jingxiu, Wang, Heng, Su, Zhiguo. Prompt and Robust Humoral Immunity Elicited by a Conjugated Chimeric Malaria Antigen with a Truncated Flagellin. BIOCONJUGATE CHEMISTRY[J]. 2018, 29(3): 761-770, http://www.irgrid.ac.cn/handle/1471x/1764212.[18] 王慧梅, 范艳敏, 王连艳. 基于微胶囊技术对油脂包埋的研究进展. 现代食品科技[J]. 2018, 34(10): 271-280+195, http://lib.cqvip.com/Qikan/Article/Detail?id=676691338.[19] 王连艳. pH-responsive Poly(D,L-Lactic-co-Glycolic Acid) Nanoparticles with Rapid Antigen Release Behavior Promote Immune Response. 第十三届全国免疫学学术大会摘要汇编null. 2018, http://ir.ipe.ac.cn/handle/122111/28909.[20] 王连艳. 靶向DC抗肿瘤疫苗制剂的研究进展. 赤子. 2018, [21] Huimei Wang, Shulan Han, Lianyan Wang, Tingyuan Yang, Guifeng Zhang, Lian Yu, Yue Zhao. Dual-function baicalin and baicalin-loaded poly(lactic-co-glycolic acid) nanoparticles: Immune activation of dendritic cells and arrest of the melanoma cell cycle at the G2/M phase. 中国颗粒学报：英文版. 2018, 64-71, http://lib.cqvip.com/Qikan/Article/Detail?id=675485769.[22] Guanghui Ma, Jie Wu, Lianyan Wang, Yueqi Wang, Qi Liu. Uniform biodegradable nanoparticles with high pH-sensitivity as vaccine adjuvant. Nanomedicine: Nanotechnology, Biology, and Medicine. 2018, 14(5): 1882-1882, http://dx.doi.org/10.1016/j.nano.2017.11.374.[23] 孟丹, 邱越佳, 杨婷媛, 王连艳, 王硕, 朱艳华, 马光辉. 聚合物脂质纳米球的制备优化. 现代生物医学进展[J]. 2018, 18(10): 1887-1891, http://lib.cqvip.com/Qikan/Article/Detail?id=675899400.[24] Yang Tingyuan, Qiu Yuejia, Jia Jilei, Lu Ting, Wang Lianyan, Ma Guanghui. Preparation and evaluation of exenatide loaded PLGA nanocapsules for oral administration. Meeting Abstractnull. 2017, 259: E128-, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000407591400222&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=3a85505900f77cc629623c3f2907beab.[25] Jia Jilei, Yang Tingyuan, Lu Ting, Liu Qi, Wang Lianyan, Ma Guanghui. Co-delivery of HBsAg and poly I: C by CaCO3 microspheres employing layer-by-layer technique. Meeting Abstractnull. 2017, 259: E63-, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000407591400105&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=3a85505900f77cc629623c3f2907beab.[26] Jia, Jilei, Zhang, Weifeng, Liu, Qi, Yang, Tingyuan, Wang, Lianyan, Ma, Guanghui. Adjuvanticity Regulation by Biodegradable Polymeric Nano/microparticle Size. MOLECULAR PHARMACEUTICS[J]. 2017, 14(1): 14-22, https://www.webofscience.com/wos/woscc/full-record/WOS:000391249000002.[27] Wang, Xing, Wu, Xia, Xing, Helin, Zhang, Guilan, Shi, Quan, E, Lingling, Liu, Na, Yang, Tingyuan, Wang, Dongsheng, Qi, Feng, Wang, Lianyan, Liu, Hongchen. Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect. ACS APPLIED MATERIALS & INTERFACES[J]. 2017, 9(13): 11380-11391, http://ir.ipe.ac.cn/handle/122111/22481.[28] 杨美洋, 王连艳, 杨婷媛, 阎雪莹. 基于可生物降解纳微颗粒的单剂免疫疫苗研究进展. 过程工程学报[J]. 2017, 17(5): 893-902, [29] Wang Lianyan, Liu Qi, Jia Jilei, Yang Tingyuan, Ma Guanghui. Design of pH-responsive poly(D, L-lactic-co-glycolic acid) nanoparticles to promote immune response. Meeting Abstractnull. 2017, 259: E81-, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000407591400138&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=3a85505900f77cc629623c3f2907beab.[30] Yang, Tingyuan, Qiu, Yuejia, Jia, Jilei, Lu, Ting, Wang, Lianyan, Ma, Guanghui. Preparation and evaluation of exenatide loaded PLGA nanocapsules for oral administration. JOURNAL OF CONTROLLED RELEASEnull. 2017, 259: E128-E129, http://dx.doi.org/10.1016/j.jconrel.2017.03.263.[31] Jia, Jilei, Yang, Tingyuan, Lu, Ting, Liu, Qi, Wang, Lianyan, Ma, Guanghui. Co-delivery of HBsAg and poly I: C by CaCO3 microspheres employing layer-by-layer technique. JOURNAL OF CONTROLLED RELEASEnull. 2017, 259: E63-E63, http://dx.doi.org/10.1016/j.jconrel.2017.03.146.[32] 马光辉. The Adjuvanticity Regulation by Biodegradable Polymeric Nano-/microparticles Size. Molecular Pharmaceutics. 2017, [33] Wang, Lianyan, Liu, Qi, Jia, Jilei, Yang, Tingyuan, Ma, Guanghui. Design of pH-responsive poly(D, L-lactic-co-glycolic acid) nanoparticles to promote immune response. JOURNAL OF CONTROLLED RELEASEnull. 2017, 259: E81-E81, http://dx.doi.org/10.1016/j.jconrel.2017.03.179.[34] Jia, Jilei, Liu, Qi, Yang, Tingyuan, Wang, Lianyan, Ma, Guanghui. Facile fabrication of varisized calcium carbonate microspheres as vaccine adjuvants. JOURNAL OF MATERIALS CHEMISTRY B[J]. 2017, 5(8): 1611-1623, http://ir.ipe.ac.cn/handle/122111/21996.[35] Zhang, Bo, Yang, Tingyuan, Wang, Qibao, Zhang, Guifeng, Huo, Junsheng, Huang, Jian, Wang, Lianyan. Fabrication of uniform alginate-agarose microcapsules loading FeSO4 using water-oil-water-oil multiple emulsions system combined with premix membrane emulsification technique. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS[J]. 2016, 498(JUNE): 128-138, http://dx.doi.org/10.1016/j.colsurfa.2016.03.039.[36] Liu, Qi, Jia, Jilei, Yang, Tingyuan, Fan, Qingze, Wang, Lianyan, Ma, Guanghui. Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses. SMALL[J]. 2016, 12(13): 1744-1757, http://ir.ipe.ac.cn/handle/122111/20982.[37] Liu, Hui, Wang, Lianyan, Yang, Tingyuan, Zhang, Guifeng, Huang, Jian, Sun, Jing, Huo, Junsheng. Optimization and evaluation of fish oil microcapsules. PARTICUOLOGY[J]. 2016, 29(DEC): 162-168, http://dx.doi.org/10.1016/j.partic.2016.04.001.[38] Yang Tingyuan, Ma Tongtong, Wang Lianyan, Wang Dong, Ma Guanghui. Uniform-sized PLGA-HSPC lipospheres as a biocompatible platform for controlled release of proteins and peptides.. Journal of controlled release : official journal of the Controlled Release Society. 2015, http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=1&recid=&FileName=SJPDA14A57F3A1B10B4138497BD2D195D630&DbName=SJPD_06&DbCode=SJPD&yx=&pr=&URLID=&bsm=.[39] Wang, Lianyan, Yang, Tingyuan, Ma, Guanghui. Particle Design of Membrane Emulsification for Protein Drug and Vaccine Delivery. CURRENT PHARMACEUTICAL DESIGN[J]. 2015, 21(19): 2563-2598, http://www.irgrid.ac.cn/handle/1471x/975477.[40] 张博, 王瑛, 王启宝, 杨婷媛, 张贵锋, 王连艳. 快速膜乳化法制备尺寸均一的载硫酸亚铁明胶微球. 过程工程学报[J]. 2015, 15(5): 847-853, http://www.irgrid.ac.cn/handle/1471x/1047928.[41] Weifeng Zhang, Lianyan Wang, Yuan Liu, Xiaoming Chen, Qi Liu, Jilei Jia, Guanghui Ma. Regulating the surface charges of polymeric microparticles to improve the efficacy of particle-adjuvanted vaccine. Journal of Controlled Release. 2015, http://dx.doi.org/10.1016/j.jconrel.2015.05.189.[42] Zhang, Weifeng, Wang, Lianyan, Yang, Tingyuan, Liu, Yuan, Chen, Xiaoming, Liu, Qi, Jia, Jilei, Ma, Guanghui. Immunopotentiator-Loaded Polymeric Microparticles as Robust Adjuvant to Improve Vaccine Efficacy. PHARMACEUTICAL RESEARCH[J]. 2015, 32(9): 2837-2850, http://www.irgrid.ac.cn/handle/1471x/979186.[43] Liu, Qi, Chen, Xiaoming, Jia, Jilei, Zhang, Weifeng, Yang, Tingyuan, Wang, Lianyan, Ma, Guanghui. pH-Responsive Poly(D,L-lactic-co-glycolic acid) Nanoparticles with Rapid Antigen Release Behavior Promote Immune Response. ACS NANO[J]. 2015, 9(5): 4925-4938, http://www.irgrid.ac.cn/handle/1471x/975485.[44] Wang, Lianyan, Cai, Mingming, Liu, Yuan, Yang, Tingyuan, Zeng, Yejing, Zhang, Yueling, Li, Qiang, Zhu, Baocheng, Ma, Guanghui. Polymer hydrophobicity regulates paclitaxel distribution in microspheres, release profile and cytotoxicity in vitro. POWDER TECHNOLOGY[J]. 2015, 275: 77-84, http://dx.doi.org/10.1016/j.powtec.2015.01.041.[45] 王连艳, 陈小明, 贾吉磊, 刘婍, 杨婷媛, 马光辉. 以纳微颗粒为佐剂的新型乙肝疫苗研发. 2015年中国化工学会年会论文集null. 2015, http://ir.ipe.ac.cn/handle/122111/20434.[46] Yang, Tingyuan, Ma, Tongtong, Wang, Lianyan, Wang, Dong, Ma, Guanghui. Uniform-sized PLGA-HSPC lipospheres as a biocompatible platform for controlled release of proteins and peptides. JOURNAL OF CONTROLLED RELEASEnull. 2015, 213(SEP): E105-E106, http://dx.doi.org/10.1016/j.jconrel.2015.05.177.[47] 王连艳. H-Responsive Poly(D,L-lactic-co-glycolicacid) Nanoparticles with Rapid Antigen Release Behavior Promote Immune Response. 2015, [48] 徐婧, 王连艳, 杨婷媛, 刘艳辉, 马光辉. 聚乳酸纳米颗粒的制备与表征. 过程工程学报[J]. 2015, 15(3): 495-500, http://www.irgrid.ac.cn/handle/1471x/1047927.[49] Zhang, Weifeng, Wang, Lianyan, Liu, Yuan, Chen, Xiaoming, Liu, Qi, Jia, Jilei, Ma, Guanghui. Regulating the surface charges of polymeric microparticles to improve the efficacy of particle-adjuvanted vaccine. JOURNAL OF CONTROLLED RELEASEnull. 2015, 213(SEP): E113-E113, http://dx.doi.org/10.1016/j.jconrel.2015.05.189.[50] Jilei Jia, Lianyan Wang, Qi Liu, Guanghui Ma. Facile Fabrication of Varisized Calcium Carbonate Microspheres as Vaccine Adjuvant. 2015, http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=3&recid=&FileName=NMZX201509001959&DbName=IPFDLAST2015&DbCode=IPFD&yx=&pr=&URLID=&bsm=.[51] 刘雁南, 王连艳, 杨婷媛, 刘艳辉, 马光辉. 白油佐剂粒径均一性与炎症和免疫学效应的相关性. 过程工程学报[J]. 2015, 15(4): 653-658, http://www.irgrid.ac.cn/handle/1471x/1047915.[52] 何超群, 杨婷媛, 王连艳, 张贵锋, 齐峰, 张嵘, 马光辉. SD大鼠II型糖尿病模型的建立与评价. 过程工程学报[J]. 2015, 15(3): 501-505, http://www.irgrid.ac.cn/handle/1471x/1047912.[53] Qiu, Shaohui, Wei, Qiang, Liang, Zhenglun, Ma, Guanghui, Wang, Lianyan, An, Wenqi, Ma, Xiaowei, Fang, Xin, He, Peng, Li, Hemin, Hu, Zhongyu. Biodegradable polylactide microspheres enhance specific immune response induced by Hepatitis B surface antigen. HUMAN VACCINES & IMMUNOTHERAPEUTICS[J]. 2014, 10(8): 2350-2356, http://www.irgrid.ac.cn/handle/1471x/944872.[54] Zhang, Weifeng, Wang, Lianyan, Liu, Yuan, Chen, Xiaoming, Liu, Qi, Jia, Jilei, Yang, Tingyuan, Qiu, Shaohui, Ma, Guanghui. Immune responses to vaccines involving a combined antigen-nanoparticle mixture and nanoparticle-encapsulated antigen formulation. BIOMATERIALS[J]. 2014, 35(23): 6086-6097, http://dx.doi.org/10.1016/j.biomaterials.2014.04.022.[55] Liu, Yuan, Wang, Lianyan, Zhang, Yu, Zhang, Weifeng, Chen, Xiaoming, Yang, Tingyuan, Wang, Zhuo, Ma, Guanghui. Uniform-sized water-in-oil vaccine formulations enhance immune response against Newcastle disease and avian influenza in chickens. INTERNATIONAL IMMUNOPHARMACOLOGY[J]. 2014, 23(2): 603-608, http://dx.doi.org/10.1016/j.intimp.2014.10.011.[56] Chen, Xiaoming, Liu, Yuying, Wang, Lianyan, Liu, Yuan, Zhang, Weifeng, Fan, Bei, Ma, Xiaowei, Yuan, Qipeng, Ma, Guanghui, Su, Zhiguo. Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg. MOLECULAR PHARMACEUTICS[J]. 2014, 11(6): 1772-1784, http://www.irgrid.ac.cn/handle/1471x/857678.[57] 王东, 王连艳, 常秀莲, 杨婷媛, 马光辉. 提高蛋白多肽口服生物利用度的方法. 过程工程学报[J]. 2014, 14(2): 354-360, http://ir.ipe.ac.cn/handle/122111/10424.[58] Nazir, H, Zhang, W, Liu, Y, Chen, X, Wang, L, Naseer, M M, Ma, G. Silicone oil emulsions: strategies to improve their stability and applications in hair care products. INTERNATIONAL JOURNAL OF COSMETIC SCIENCEnull. 2014, 36(2): 124-133, http://www.irgrid.ac.cn/handle/1471x/837103.[59] Ma, Tongtong, Wang, Lianyan, TingyuanYang, Wang, Dong, Ma, Guanghui, Wang, Siling. PLGA-lipid liposphere as a promising platform for oral delivery of proteins. COLLOIDS AND SURFACES B-BIOINTERFACES[J]. 2014, 117: 512-519, http://dx.doi.org/10.1016/j.colsurfb.2014.02.039.[60] Ma, Tongtong, Wang, Lianyan, Yang, Tingyuan, Ma, Guanghui, Wang, Siling. M-cell targeted polymeric lipid nanoparticles containing a toll-like receptor agonist to boost oral immunity. INTERNATIONAL JOURNAL OF PHARMACEUTICS[J]. 2014, 473(1-2): 296-303, http://www.irgrid.ac.cn/handle/1471x/944870.[61] Dai, Lin, Yang, Tingyuan, He, Jing, Deng, Lihong, Liu, Jing, Wang, Luying, Lei, Jiandu, Wang, Lianyan. Cellulose-graft-poly(L-lactic acid) nanoparticles for efficient delivery of anti-cancer drugs. JOURNAL OF MATERIALS CHEMISTRY B[J]. 2014, 2(39): 6749-6757, http://www.irgrid.ac.cn/handle/1471x/944860.[62] 王连艳. Synthesis characterization and adsorption behavior of molecularly imprinted nanoparticles for tripterine using precipitation polymerization. 2014, [63] Zhang, Weifeng, Wang, Lianyan, Liu, Yuan, Chen, Xiaoming, Li, Jiahui, Yang, Tingyuan, An, Wenqi, Ma, Xiaowei, Pan, Ruowen, Ma, Guanghui. Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis. PHARMACEUTICAL RESEARCH[J]. 2014, 31(4): 1015-1031, http://www.irgrid.ac.cn/handle/1471x/836958.[64] Liu, Yuying, Chen, Xiaoming, Wang, Lianyan, Yang, Tingyuan, Yuan, Qipeng, Ma, Guanghui. Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro. PARTICUOLOGY[J]. 2014, 17(DEC): 74-80, http://lib.cqvip.com/Qikan/Article/Detail?id=663472691.[65] Liu, Yuan, Zhang, Guifeng, Deng, Lihong, Lei, Jiandu, Wang, Lianyan, He, Jing. Synthesis, characterization and adsorption performance of molecularly imprinted nanoparticles for tripterine by precipitation polymerization. ANALYTICAL METHODS[J]. 2014, 6(3): 684-689, http://www.irgrid.ac.cn/handle/1471x/837126.[66] Chen, Xiaoming, Wang, Lianyan, Liu, Qi, Jia, Jilei, Liu, Yuan, Zhang, Weifeng, Ma, Guanghui, Su, Zhiguo. Polycation-decorated PLA microspheres induce robust immune responses via commonly used parenteral administration routes. INTERNATIONAL IMMUNOPHARMACOLOGY[J]. 2014, 23(2): 592-602, http://dx.doi.org/10.1016/j.intimp.2014.10.010.[67] Yuying Liu, Xiaoming Chen, Lianyan Wang, Tingyuan Yang, Qipeng Yuan, Guanghui Ma. Surface charge of PLA microparticles in regulation of antigen loading,macrophage phagocytosis and activation,and immune effects in vitro. 中国颗粒学报：英文版. 2014, 74-80, http://lib.cqvip.com/Qikan/Article/Detail?id=663472691.[68] Tongtong Ma, Lianyan Wang, Tingyuan Yang, Guanghui Ma, Siling Wang. Homogeneous PLGA-lipid nanoparticle as a promising oral vaccine delivery system for ovalbumin. ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES[J]. 2014, 9(3): 129-136, http://dx.doi.org/10.1016/j.ajps.2014.03.002.[69] Liu, Yuan, Yin, Ying, Wang, Lianyan, Zhang, Weifeng, Chen, Xiaoming, Yang, Xiaoxiao, Xu, Junjie, Ma, Guanghui. Surface hydrophobicity of microparticles modulates adjuvanticity. JOURNAL OF MATERIALS CHEMISTRY B[J]. 2013, 1(32): 3888-3896, http://ir.ipe.ac.cn/handle/122111/13360.[70] Wang, Lianyan, Liu, Yuan, Zhang, Weifeng, Chen, Xiaoming, Yang, Tingyuan, Ma, Guanghui. Microspheres and Microcapsules for Protein Delivery: Strategies of Drug Activity Retention. CURRENT PHARMACEUTICAL DESIGN[J]. 2013, 19(35): 6340-6352, http://ir.ipe.ac.cn/handle/122111/13549.[71] 贾成成, 王连艳, 方颖, 陈健平, 曹科, 刘永江, 马光辉. PLGA微球粒径对HPV L1五聚体候选疫苗免疫效果的调控. 《过程工程学报》[J]. 2013, 13(4): 638-645, http://www.irgrid.ac.cn/handle/1471x/856778.[72] Liu, Yuan, Yin, Ying, Wang, Lianyan, Zhang, Weifeng, Chen, Xiaoming, Yang, Xiaoxiao, Xu, Junjie, Ma, Guanghui. Engineering Biomaterial-Associated Complement Activation to Improve Vaccine Efficacy. BIOMACROMOLECULES[J]. 2013, 14(9): 3321-3328, http://ir.ipe.ac.cn/handle/122111/13486.[73] Wang, Lianyan, Yang, Tingyuan, Yang, Jian, Li, Qiang, Hao, Dongxia, Yang, Juanjuan, Ma, Guanghui. In situ magnetization technique for synthesis of magnetic polymer microspheres. POWDER TECHNOLOGY[J]. 2013, 235: 1017-1024, http://dx.doi.org/10.1016/j.powtec.2012.12.014.[74] 刘玉莹, 陈小明, 王连艳, 袁其朋, 马光辉. 鱼精蛋白对PLA微球佐剂的调控. 过程工程学报[J]. 2013, 13(4): 646-653, http://www.irgrid.ac.cn/handle/1471x/857006.[75] Li, Qiang, Zhang, Rongyue, Li, Juan, Yan, Xiaofeng, Wang, Lianyan, Gong, Fangling, Su, Zhiguo, Ma, Guanghui. In situ inhibitor (HCl) removal promoted heterogeneous Friedel-Crafts reaction of polystyrene microsphere with Lewis acids catalysts. JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL[J]. 2013, 370: 56-63, http://dx.doi.org/10.1016/j.molcata.2012.12.009.[76] Yue, Hua, Wei, Wei, Fan, Bei, Yue, Zhanguo, Wang, Lianyan, Ma, Guanghui, Su, Zhiguo. The orchestration of cellular and humoral responses is facilitated by divergent intracellular antigen trafficking in nanoparticle-based therapeutic vaccine. PHARMACOLOGICAL RESEARCH[J]. 2012, 65(2): 189-197, http://dx.doi.org/10.1016/j.phrs.2011.09.008.[77] 蔡明明, 王连艳, 吕丕平, 马光辉, 朱宝成. 壳聚糖季铵盐表面修饰对载紫杉醇PLGA微球体外药效学的影响. 过程工程学报. 2012, 12(3): 454-459, http://lib.cqvip.com/Qikan/Article/Detail?id=42299644.[78] 刘茜, 张娟, 陈坚, 张岳玲, 王连艳, 王淼, 马光辉, 堵国成. 喷雾冷凝法制备高性能乳酸菌微胶囊. 应用与环境生物学报[J]. 2012, 18(5): 824-830, http://www.irgrid.ac.cn/handle/1471x/856982.[79] Nazir, Habiba, Wang, Lianyan, Lian, Guoping, Zhu, Shiping, Zhang, Yueling, Liu, Yuan, Ma, Guanghui. Multilayered silicone oil droplets of narrow size distribution: Preparation and improved deposition on hair. COLLOIDS AND SURFACES B-BIOINTERFACES[J]. 2012, 100: 42-49, http://dx.doi.org/10.1016/j.colsurfb.2012.05.018.[80] Zhang, Yueling, Wei, Wei, Lv, Piping, Wang, Lianyan, Ma, Guanghui. Preparation and evaluation of alginate-chitosan microspheres for oral delivery of insulin. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS[J]. 2011, 77(1): 11-19, http://dx.doi.org/10.1016/j.ejpb.2010.09.016.[81] 王连艳. Fabrication strategy for amphiphilic microcapsules with narrow size distribution by premix membrane emulsi?cation. Colloids and Surfaces B: Biointerfaces. 2011, [82] Zhang, Yueling, Jia, Xingyuan, Wang, Lianyan, Liu, Jingzhong, Ma, Guanghui. Preparation of Ca-Alginate Microparticles and Its Application for Phenylketonuria Oral Therapy. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH[J]. 2011, 50(7): 4106-4112, http://www.irgrid.ac.cn/handle/1471x/735951.[83] Yue, ZhanGuo, Wei, Wei, Lv, PiPing, Yue, Hua, Wang, LianYan, Su, ZhiGuo, Ma, GuangHui. Surface Charge Affects Cellular Uptake and Intracellular Trafficking of Chitosan-Based Nanoparticles. BIOMACROMOLECULES[J]. 2011, 12(7): 2440-2446, http://www.irgrid.ac.cn/handle/1471x/735937.[84] Lv, PiPing, Wei, Wei, Yue, Hua, Yang, TingYuan, Wang, LianYan, Ma, GuangHui. Porous Quaternized Chitosan Nanoparticles Containing Paclitaxel Nanocrystals Improved Therapeutic Efficacy in Non-Small-Cell Lung Cancer after Oral Administration. BIOMACROMOLECULES[J]. 2011, 12(12): 4230-4239, http://www.irgrid.ac.cn/handle/1471x/735900.[85] Nazir, Habiba, Lv, Piping, Wang, Lianyan, Lian, Guoping, Zhu, Shiping, Ma, Guanghui. Uniform-sized silicone oil microemulsions: Preparation, investigation of stability and deposition on hair surface. JOURNAL OF COLLOID AND INTERFACE SCIENCE[J]. 2011, 364(1): 56-64, http://dx.doi.org/10.1016/j.jcis.2011.07.096.[86] Wei, Yi, Wang, Yuxia, Wang, Lianyan, Hao, Dongxia, Ma, Guanghui. Fabrication strategy for amphiphilic microcapsules with narrow size distribution by premix membrane emulsification. COLLOIDS AND SURFACES B-BIOINTERFACES[J]. 2011, 87(2): 399-408, http://dx.doi.org/10.1016/j.colsurfb.2011.05.051.[87] 宋春艳, 王连艳, 吴有斌, 张竞, 马光辉, 张栩. 壳聚糖微球的自发荧光机理. 过程工程学报[J]. 2011, 11(3): 487-491, http://www.jproeng.com/CN/Y2011/V11/I3/487.[88] Wei, Wei, Ma, GuangHui, Wang, LianYan, Wu, Jie, Su, ZhiGuo. Hollow quaternized chitosan microspheres increase the therapeutic effect of orally administered insulin. ACTA BIOMATERIALIA[J]. 2010, 6(1): 205-209, http://dx.doi.org/10.1016/j.actbio.2009.06.005.[89] Yue, Hua, Wei, Wei, Yue, Zhanguo, Lv, Piping, Wang, Lianyan, Ma, Guanghui, Su, Zhiguo. Particle size affects the cellular response in macrophages. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES[J]. 2010, 41(5): 650-657, http://dx.doi.org/10.1016/j.ejps.2010.09.006.[90] 马光辉, 王连艳, 魏炜, 吴颉, 张岳玲, 吕坯平, 苏志国. 用于蛋白质多肽药物给药的壳聚糖微球的制备和药物活性保持策略. 2010年全国高分子材料科学与工程研讨会学术论文集（上册）null. 2010, 2-, http://www.irgrid.ac.cn/handle/1471x/717276.[91] Yang, Jian, Hao, DongXia, Bi, CaiXia, Su, ZhiGuo, Wang, LianYan, Ma, GuangHui. Rapid Synthesis of Uniform Magnetic Microspheres by Combing Premix Membrane Emulsification and in Situ Formation Techniques. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH[J]. 2010, 49(13): 6047-6053, http://www.irgrid.ac.cn/handle/1471x/779174.[92] 王连艳, 马光辉. 尺寸均一的微球和微囊的制备及作为药物载体的应用. 生物颗粒与粉体制备、应用技术研讨会论文集null. 2010, 2-, http://www.irgrid.ac.cn/handle/1471x/717331.[93] Ma GuangHui, Yang Jian, Lv PiPing, Wang LianYan, Wei Wei, Tian Rui, Wu Jie, Su ZhiGuo. Preparation of Uniform Microspheres and Microcapsules by Modified Emulsification Process. MACROMOLECULAR SYMPOSIAnull. 2010, 288: 41-48, [94] 曾烨婧, 王连艳, 马光辉, 马润宇. 快速膜乳化法制备载紫杉醇聚乳酸类微球. 过程工程学报[J]. 2010, 10(3): 568-575, http://lib.cqvip.com/Qikan/Article/Detail?id=34454614.[95] 李娜, 王连艳, 吴有斌, 曾烨婧, 马光辉, 苏志国. 乙肝表面抗原在微球表面的吸附行为、活性和结构变化. 过程工程学报[J]. 2010, 10(3): 559-567, http://lib.cqvip.com/Qikan/Article/Detail?id=34454613.[96] 岳华, 魏炜, 岳占国, 吕丕平, 王连艳, 马光辉, 苏志国. 纳微球粒径大小对免疫学效应的影响. 生物颗粒与粉体制备、应用技术研讨会论文集null. 2010, 2-, http://www.irgrid.ac.cn/handle/1471x/717398.[97] Lv, PiPing, Wei, Wei, Gong, FangLing, Zhang, YueLing, Zhao, HuiYing, Lei, JianDu, Wang, LianYan, Ma, GuangHui. Preparation of Uniformly Sized Chitosan Nanospheres by a Premix Membrane Emulsification Technique. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH[J]. 2009, 48(19): 8819-8828, http://www.irgrid.ac.cn/handle/1471x/778910.[98] 王连艳. 利用快速膜乳化法制备粒径均一的PLGA纳微球和纳微囊. 过程工程学报. 2009, [99] 田瑞, 王连艳, 吴颉, 张洁, 曾烨婧, 马光辉. 快速膜乳化法制备粒径均一的PLGA微球和微囊. 过程工程学报[J]. 2009, 754-762, http://lib.cqvip.com/Qikan/Article/Detail?id=31516115.[100] Zhou, QingZhu, Wang, LianYan, Ma, GuangHui, Su, ZhiGuo. Multi-stage premix membrane emulsification for preparation of agarose microbeads with uniform size. JOURNAL OF MEMBRANE SCIENCE[J]. 2008, 322(1): 98-104, http://dx.doi.org/10.1016/j.memsci.2008.05.025.[101] Wei, Qiang, Wei, Wei, Tian, Rui, Wang, Lianyan, Su, ZhiGuo, Ma, GuangHui. Preparation of uniform-sized PELA microspheres with high encapsulation efficiency of antigen by premix membrane emulsification. JOURNAL OF COLLOID AND INTERFACE SCIENCE[J]. 2008, 323(2): 267-273, http://dx.doi.org/10.1016/j.jcis.2008.04.058.[102] Zhang, Yueling, Lian, Guoping, Zhu, Shiping, Wang, Lianyan, Wei, Wei, Ma, Guanghui. Investigation on the uniformity and stability of sunflower oil/water emulsions prepared by a shirasu porous glass membrane. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH[J]. 2008, 47(17): 6412-6417, http://www.irgrid.ac.cn/handle/1471x/725410.[103] Wei, Qiang, Wei, Wei, Lai, Bo, Wang, LianYan, Wang, YuXia, Su, ZhiGuo, Ma, GuangHui. Uniform-sized PLA nanoparticles: Preparation by premix membrane emulsification. INTERNATIONAL JOURNAL OF PHARMACEUTICS[J]. 2008, 359(1-2): 294-297, http://www.irgrid.ac.cn/handle/1471x/725369.[104] Wei Wei, Wang LianYan, Yuan Lan, Yang XiaoDa, Su ZhiGuo, Ma GuangHui. Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration.. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. 2008, http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=1&recid=&FileName=SJPD12102102490040&DbName=SJPD_02&DbCode=SJPD&yx=&pr=&URLID=&bsm=.[105] Wei, Wei, Yuan, Lan, Hu, Gang, Wang, LianYan, Wu, He, Hu, Xue, Su, ZhiGuo, Ma, GuangHui. Monodisperse chitosan microspheres with interesting structures for protein drug delivery. ADVANCED MATERIALS[J]. 2008, 20(12): 2292-+, http://www.irgrid.ac.cn/handle/1471x/725373.[106] Wei, Wei, Wang, LianYan, Wu, Jie, Ma, GuangHui. Quaternized chitosan microspheres as insulin carrier. JOURNAL OF BIOTECHNOLOGYnull. 2008, 136: S452-S452, http://dx.doi.org/10.1016/j.jbiotec.2008.07.1050.[107] Wei, Wei, Wang, LianYan, Yuan, Lan, Yang, XiaoDa, Su, ZhiGuo, Ma, GuangHui. Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS[J]. 2008, 69(3): 878-886, http://dx.doi.org/10.1016/j.ejpb.2008.02.011.[108] Wu, Jie, Wei, Wei, Wang, LianYan, Su, ZhiGuo, Ma, GuangHui. Preparation of uniform-sized pH-sensitive quaternized chitosan microsphere by combining membrane emulsification technique and thermal-gelation method. COLLOIDS AND SURFACES B-BIOINTERFACES[J]. 2008, 63(2): 164-175, http://dx.doi.org/10.1016/j.colsurfb.2007.11.021.[109] Wu, Jie, Wei, Wei, Wang, LianYan, Su, ZhiGuo, Ma, GuangHui. A thermosensitive hydrogel based on quaternized chitosan and poly(ethylene glycol) for nasal drug delivery system. BIOMATERIALS[J]. 2007, 28(13): 2220-2232, http://dx.doi.org/10.1016/j.biomaterials.2006.12.024.[110] Zhou, QingZhu, Wang, LianYan, Ma, GuangHui, Su, ZhiGuo. Preparation of uniform-sized agarose beads by microporous membrane emulsification technique. JOURNAL OF COLLOID AND INTERFACE SCIENCE[J]. 2007, 311(1): 118-127, http://dx.doi.org/10.1016/j.jcis.2007.02.040.[111] Wei, Wei, Wang, LianYan, Yuan, Lan, Wei, Qiang, Yang, XiaoDa, Su, ZhiGuo, Ma, GuangHui. Preparation and application of novel microspheres possessing autofluorescent properties. ADVANCED FUNCTIONAL MATERIALS[J]. 2007, 17(16): 3153-3158, http://www.irgrid.ac.cn/handle/1471x/725801.[112] 王连艳. 尺寸均一的壳聚糖载药微球制备及应用研究. 2006, 175-, http://ir.ipe.ac.cn/handle/122111/1008.[113] Wang, LianYan, Gu, YongHong, Zhou, QingZhu, Ma, GuangHui, Wan, YinHua, Su, ZhiGuo. Preparation and characterization of uniform-sized chitosan microspheres containing insulin by membrane emulsification and a two-step solidification process. COLLOIDS AND SURFACES B-BIOINTERFACES[J]. 2006, 50(2): 126-135, http://dx.doi.org/10.1016/j.colsurfb.2006.05.006.[114] 古永红, 王连艳, 谭天伟, 马光辉. 尺寸均一的壳聚糖微球的制备及其作为胰岛素控释载体的研究. 生物工程学报. 2006, 22(1): 150-155, http://lib.cqvip.com/Qikan/Article/Detail?id=21193009.[115] 王连艳, 魏炜, 马光辉, 苏志国. 尺寸均一、高活性保持的壳聚糖载药微球的制备. 第三届全国化学工程与生物化工年会论文摘要集（下）null. 2006, 1-, http://www.irgrid.ac.cn/handle/1471x/717332.[116] Wang, LY, Gu, YH, Su, ZG, Ma, GH. Preparation and improvement of release behavior of chitosan microspheres containing insulin. INTERNATIONAL JOURNAL OF PHARMACEUTICS[J]. 2006, 311(1-2): 187-195, http://www.irgrid.ac.cn/handle/1471x/736301.[117] Wang, LY, Ma, GH, Su, ZG. Preparation of uniform sized chitosan microspheres by membrane emulsification technique and application as a carrier of protein drug. JOURNAL OF CONTROLLED RELEASE[J]. 2005, 106(1-2): 62-75, http://dx.doi.org/10.1016/j.jconrel.2005.04.005.[118] 王连艳, 马光辉. 疫苗控释微球研究进展. 国外医学：预防．诊断．治疗用生物制品分册. 2004, 27(2): 78-83, http://lib.cqvip.com/Qikan/Article/Detail?id=9432515.[119] 王连艳, 魏国侠. 采用挤压—扩散置换设备提高麦草浆黑液提取率. 中国造纸[J]. 2002, 21(3): 41-43, http://lib.cqvip.com/Qikan/Article/Detail?id=6252356.[120] 于钢, 王连艳, 钱学艳, 刘文波. 壳聚糖作无碳复写纸用微囊壁材. 中国造纸[J]. 2002, 21(1): 19-22, http://lib.cqvip.com/Qikan/Article/Detail?id=6206117.[121] 王连艳, 钱学仁. 微胶囊技术及其在造纸上的应用. 黑龙江造纸. 1999, 6-9, http://lib.cqvip.com/Qikan/Article/Detail?id=3839220.
发表著作
（1） 生物材料科学里几丁质和壳聚糖的目前研究和进展：壳聚糖微球的制备和应用, “Current Research and Developments on Chitin and Chitosan in Biomaterial Science”, Preparation and Applications of Chitosan Microsphere, Research Signpost, 2008-07, 第 1 作者（2） 生物技术中的微球和微囊：设计，制备和应用, Microspheres and Microcapsules for Protein Drug Delivery, published in “Microspheres and Microcapsules in Biotechnology: Design, Preparation and Applications” , Pan Stanford Publishing, 2012-10, 第 1 作者（3） 海藻酸盐：生产，类型和应用, Alginates: production, types and applications, published in “Alginates: Production, Types and Applications”, , Nova Science Publishers, Inc, 2012-12, 第 1 作者
科研活动
科研项目
（ 1 ） 新型纳微球佐剂治疗性乙肝疫苗研制, 主持, 国家级, 2011-01--2012-12（ 2 ） 纳米技术增加难溶药物功效的生物安全性研究, 参与, 国家级, 2009-01--2013-12（ 3 ） 纳微颗粒佐剂剂型化研究, 主持, 国家级, 2012-01--2015-12（ 4 ） 新型纳微球佐剂治疗性乙肝疫苗研制, 主持, 国家级, 2011-01--2012-12（ 5 ） 免疫原递送微粒载体构建与递呈机制, 参与, 国家级, 2013-01--2017-12（ 6 ） 聚乳酸纳微球作为宫颈癌疫苗微球制剂的研究, 主持, 国家级, 2014-01--2016-12（ 7 ） 疫苗新佐剂与新剂型的研发, 主持, 部委级, 2011-01--2014-12（ 8 ） 动物疫苗制剂及下游工艺技术研究与示范, 主持, 国家级, 2013-01--2017-12（ 9 ） 多功能“薄皮大腔”PLGA 纳微囊佐剂的构建及其作用机制 , 主持, 国家级, 2015-01--2018-12（ 10 ） 聚乳酸纳微球佐剂的乙肝疫苗制剂开发, 主持, 院级, 2014-06--2016-06（ 11 ） 聚乳酸类纳米颗粒疫苗佐剂关键技术研究, 主持, 国家级, 2016-01--2020-12（ 12 ） 模式与特色动物实验平台, 主持, 部委级, 2016-01--2020-12（ 13 ） 基于肿瘤微环境的时空次序递送VLP纳米疫苗构建及免疫治疗相关研究, 主持, 国家级, 2020-01--2023-12（ 14 ） 基于碳酸盐颗粒的VLP疫苗递送体系与免疫治疗应用基础研究, 主持, 国家级, 2020-07--2023-06
参与会议
（1）基于DC靶向的“薄皮大腔” PLGA纳微囊的肿瘤免疫 2019 生物治疗产业大会 2019-03-23（2）嗜酸乳杆菌对肠道微生态及口服免疫的影响 食品与功能国际研讨会 王连艳，张宇 2018-05-25（3）Immune responses to vaccines involving a combined antigen-nanoparticle mixture and nanoparticle-encapsulated antigen formulation 2015-12-06（4）PLA Microspheres Induce Robust Immune Responses via Several Commonly Used Parenteral Administration Routes 2015-10-25（5）以纳微颗粒为佐剂的新型乙肝疫苗研发 中国化工年会 2015-10-17（6）pH-Responsive Poly(D, L-lactic-co-glycolic acid) Nanoparticles with Rapid Antigen Release Behavior Promote Immune Response 第五届中国药物制剂大会 2015-09-25（7）PLA Microspheres Induce Robust Immune Responses via Several Commonly Used Parenteral Administration Routes 2015-09-03（8）膜乳化技术及其在食品微包埋中的应用 营养食品新技术学术会议 2015-01-25（9）Preparation of Uniform-sized Polysaccharide Microspheres with Novel Structure and Application as Oral Delivery System for Insulin. Lianyan Wang, Wei Wei, Yueling Zhang, Guanghui Ma. 2011-05-29（10）尺寸均一聚合物纳微球制备及应用 中国微米纳米技术学会纳米科学技术分会纳米生物与医药技术专业委员会2010学术年会 王连艳，吕丕平，岳华，刘媛，马光辉 2010-11-26（11）Preparation of Uniform-sized Mico- and Nano-particles and Their Applications in Drug Delivery Systems. Lianyan Wang, Wei Wei, Yueling Zhang, Yuan Liu, Guanghui Ma. 2010-10-23（12）尺寸均一的微球和微囊的制备及作为药物载体的应用 生物颗粒与粉体制备、应用技术研讨会 王连艳，马光辉 2010-09-27（13）尺寸均一的自发荧光壳聚糖微球制备与应用， 全国第八届海洋生物技术与创新药物学术讨论会 王连艳，魏炜，吕丕平，马光辉 2010-08-08（14） Preparation and Application of ALG/CHI Microspheres with Narrow Size Distribution for Oral Delivery of Insulin. L.-Y. Wang, Y.-L. Zhang, W. Wei, G.-H. Ma. 2010-05-22（15）Effect of particle size in macrophage responses. Lianyan Wang, Hua Yue, Wei Wei, Guanghui Ma. 2010-05-22（16）尺寸均一、可控纳微载体制备与应用， 全国生物材料大会 王连艳，马光辉 2010-04-15（17）Monodisperse Chitosan Microspheres with Interesting Structures for Proein Drug Delivery Lianyan Wang, Wei Wei, Guanghui Ma, Zhiguo Su 2009-10-22（18）Preparation of Uniform-sized Chitosan Microspheres with Autofluorescence and Its Application in Vivo Lianyan Wang, Wei Wei, Jie Wu, Yueling Zhang, Guanghui Ma, Zhiguo Su 2009-09-24（19）Preparation of Uniform-sized Chitosan Microspheres with Autofluorescence and Its Application in Vivo Lian-Yan Wang, Wei Wei, Jie Wu, Yue-Ling Zhang, Guang-Hui Ma, Zhi-guo Su 2009-04-26（20）Preparation of Uniform Chitosan Micro- and Nano-spheres by Membrane Emulsification Technique Lian-Yan Wang, Pi-Ping Lv, Wei Wei, Yue-Ling Zhang, Jie Wu, Guang-Hui Ma 2008-12-09（21）Autofluorescence of Chitosan Microspheres and Its Application in Vivo Lianyan Wang, Wei Wei, Guanggui Ma. 2006-11-25（22）尺寸均一、高活性保持的壳聚糖载药微球的制备 第三届全国化学工程与生物化工年会 王连艳，魏炜，马光辉，苏志国 2006-11-10（23）Preparation and Characterization of Uniform-sized Chitosan Microspheres Containing Bioactive Drug Lian-Yan Wang, Guang-Hui Ma 2005-10-24（24）尺寸均一的壳聚糖载药微球的制备和应用 第三届海洋生物高技术论坛论文集 王连艳，马光辉，苏志国 2005-08-22（25）尺寸均一的壳聚糖微球的制备 第一届全国化学工程与生物化工年会 王连艳，孟凡涛，马光辉 2004-11-05
2013 中国科学院大学，网络信息中心.